Abstract. The biological nitrogen cycle is a complex interplay between many microorganisms catalyzing different reactions. For a long time, ammonia and nitrite

Ammonia-oxidizing bacteria (AOB), which oxidize ammonia to nitrite in the first step of nitrification, play an important role in biological wastewater treatment systems. Nitrosomonas mobilis is an important and dominant AOB in various wastewater treatment systems. However, the detailed physiological and genomic properties of N. mobilis have not been thoroughly investigated because of limited success isolating pure cultures. This study investigated the key physiological characteristics of N. mobilis Ms1, which was previously isolated into pure culture from the nitrifying granules of wastewater treatment bioreactor. The pure culture of N. mobilis Ms1 was cultivated in liquid mineral medium with 30 mg-N L-1 (2.14 mM) of ammonium at room temperature under dark conditions. The optimum growth of N. mobilis Ms1 occurred at 27°C and pH 8, with a maximum growth rate of 0.05–0.07 h-1, which corresponded to a generation time of 10–14 h. The half saturation constant for ammonium uptake rate and the maximum ammonium uptake rate of N. mobilis Ms1 were 30.70±0.51 μM NH4+ and 0.01±0.002 pmol NH4+ cells-1 h-1, respectively. N. mobilis Ms1 had higher ammonia oxidation activity than N. europaea in this study. The oxygen uptake activity kinetics of N. mobilis Ms1 were K_(m(O_2))= 21.74±4.01 μM O2 and V_(max⁡(O_2))= 0.06±0.02 pmol O2 cells-1 h-1. Ms1 grew well at ammonium and NaCl concentrations of up to 100 mM and 500 mM, respectively. The nitrite tolerance of N. mobilis Ms1 was extremely high (up to 300 mM) compared to AOB previously isolated from activated sludge and wastewater treatment plants. The average nucleotide identity between the genomes of N. mobilis Ms1 and other Nitrosomonas species indicated that N. mobilis Ms1 was distantly related to other Nitrosomonas species. The organization of the genes encoding protein inventory involved in ammonia oxidation and nitrifier denitrification processes were different from other Nitrosomonas species. The current study provides a needed physiological and genomic characterization of N. mobilis-like bacteria and a better understanding of their ecophysiological properties, enabling comparison of these bacteria with other AOB in wastewater treatment systems and natural ecosystems.

Nitrosomonas sp. Is79 is a chemolithoautotrophic ammonia-oxidizing bacterium that belongs to the family Nitrosomonadaceae within the phylum Proteobacteria. Ammonia oxidation is the first step of nitrification, an important process in the global nitrogen cycle ultimately resulting in the production of nitrate. Nitrosomonas sp. Is79 is an ammonia oxidizer of high interest because it is adapted to low ammonium and can be found in freshwater environments around the world. The 3,783,444-bp chromosome with a total of 3,553 protein coding genes and 44 RNA genes was sequenced by the DOE-Joint Genome Institute Program CSP 2006.

nitrifying bacterium, plural Nitrifying Bacteria, any of a small group of aerobic bacteria (family Nitrobacteraceae) that use inorganic chemicals as an energy source. They are microorganisms that are important in the nitrogen cycle as converters of soil ammonia to nitrates, compounds usable by plants. The nitrification process requires the mediation of two distinct groups: bacteria that convert ammonia to nitrites (Nitrosomonas, Nitrosospira, Nitrosococcus, and Nitrosolobus) and bacteria that convert nitrites (toxic to plants) to nitrates (Nitrobacter, Nitrospina, and Nitrococcus). In agriculture, irrigation with dilute solutions of ammonia results in an increase in soil nitrates through the action of nitrifying bacteria.

Nitrification Nitrification is the process by which ammonia is converted to nitrites (NO 2 -) and th

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Learn about nitrification. See the nitrogen cycle, explore the nitrification process, and understand factors, chemical equations, and bacteria in...

Objective In this lesson we will answer the following questions: How nitrogen is broken down. What i

Nitrosomonas convert ammonia into nitrates by a process called nitrification. It is performed in two steps - nitrite formation and nitrate formation.

Nitrification is the sequential conversion of ammonia to nitrite and ultimately nitrate

This is a curated page. Report corrections to Microbewiki. A Microbial Biorealm page on the genus Ni

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