Abe, F., Nagafuji, S., Yamauchi, T., Okabe, H., Maki, J., Higo, H., Akahane, H., Aguilar, A., Jimenez-Estrada, M., and Reyes-Chilpa, R. Trypanocidal constituents in plants 1. Evaluation of some Mexican plants for their trypanocidal activity and active constituents in Guaco, roots of Aristolochia taliscana. Biol Pharm Bull 2002;25(9):1188-1191. View abstract.
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Affuso, F., Ruvolo, A., Micillo, F., Sacca, L., and Fazio, S. Effects of a nutraceutical combination (berberine, red yeast rice and policosanols) on lipid levels and endothelial function randomized, double-blind, placebo-controlled study. Nutr Metab Cardiovasc.Dis 2010;20(9):656-661. View abstract.
Albal, M. V., Jadhav, S., and Chandorkar, A. G. Clinical evaluation of berberine in mycotic infections. Indian J Ophthalmol. 1986;34:91-92. View abstract.
Arana, B. A., Navin, T. R., Arana, F. E., Berman, J. D., and Rosenkaimer, F. Efficacy of a short course (10 days) of high-dose meglumine antimonate with or without interferon-gamma in treating cutaneous leishmaniasis in Guatemala. Clin Infect Dis 1994;18(3):381-384. View abstract.
Babbar, O. P., Chhatwal, V. K., Ray, I. B., and Mehra, M. K. Effect of berberine chloride eye drops on clinically positive trachoma patients. Indian J Med Res. 1982;76 Suppl:83-88. View abstract.
Berberine. Altern Med Rev 2000;5(2):175-177. View abstract.
Carlomagno, G., Pirozzi, C., Mercurio, V., Ruvolo, A., and Fazio, S. Effects of a nutraceutical combination on left ventricular remodeling and vasoreactivity in subjects with the metabolic syndrome. Nutr Metab Cardiovasc.Dis 2012;22(5):e13-e14. View abstract.
Chae, S. H., Jeong, I. H., Choi, D. H., Oh, J. W., and Ahn, Y. J. Growth-inhibiting effects of Coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria. J Agric.Food Chem 1999;47(3):934-938. View abstract.
Chekalina, S. I., Umurzakova, R. Z., Saliev, K. K., and Abdurakhmanov, T. R. [Effect of berberine bisulfate on platelet hemostasis in thrombocytopenia patients]. Gematologiia i Transfuziologiia 1994;39(5):33-35. View abstract.
Cheng, Z., Pang, T., Gu, M., Gao, A. H., Xie, C. M., Li, J. Y., Nan, F. J., and Li, J. Berberine-stimulated glucose uptake in L6 myotubes involves both AMPK and p38 MAPK. Biochim.Biophys.Acta 2006;1760(11):1682-1689. View abstract.
Choudhry, V. P., Sabir, M., and Bhide, V. N. Berberine in giardiasis. Indian Pediatr. 1972;9(3):143-146. View abstract.
Chun YT, Yip TT, Lau KL, and et al. A biochemical study on the hypotensive effect of berberine in rats. Gen Pharmac 1979;10:177-182. View abstract.
Chung JG, Wu LT, Chang SH, and et al. Inhibitory actions of berberine on growth and arylamine N-acetyltransferase activity in strains of Helicobacter Pylori from peptic ulcer patients. International Journal of Toxicology 1999;18:35.
Chung, J. G., Chen, G. W., Hung, C. F., Lee, J. H., Ho, C. C., Ho, H. C., Chang, H. L., Lin, W. C., and Lin, J. G. Effects of berberine on arylamine N-acetyltransferase activity and 2-aminofluorene-DNA adduct formation in human leukemia cells. Am J Chin Med 2000;28(2):227-238. View abstract.
Chung, J. G., Wu, L. T., Chu, C. B., Jan, J. Y., Ho, C. C., Tsou, M. F., Lu, H. F., Chen, G. W., Lin, J. G., and Wang, T. F. Effects of berberine on arylamine N-acetyltransferase activity in human bladder tumour cells. Food Chem Toxicol 1999;37(4):319-326. View abstract.
Cianci, A., Cicero, A. F., Colacurci, N., Matarazzo, M. G., and De, Leo, V. Activity of isoflavones and berberine on vasomotor symptoms and lipid profile in menopausal women. Gynecol.Endocrinol. 2012;28(9):699-702. View abstract.
Desai, A. B., Shah, K. M., and Shah, D. M. Berberine in treatment of diarrhoea. Indian Pediatr. 1971;8(9):462-465. View abstract.
Dutta NK and Panse MV. Usefulness of berberine (an alkaloid from Berberis aristata) in the treatment of cholera (experimental). Indian J Med Res 1962;50(5):732-736.
Freile, M. L., Giannini, F., Pucci, G., Sturniolo, A., Rodero, L., Pucci, O., Balzareti, V., and Enriz, R. D. Antimicrobial activity of aqueous extracts and of berberine isolated from Berberis heterophylla. Fitoterapia 2003;74(7-8):702-705. View abstract.
Fukuda, K., Hibiya, Y., Mutoh, M., Koshiji, M., Akao, S., and Fujiwara, H. Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells. J Ethnopharmacol. 1999;66(2):227-233. View abstract.
Ghosh AK, Bhattacharyya FK, and Ghosh DK. Leishmania donovani: amastigote inhibition and mode of action of berberine. Experimental Parasitology 1985;60:404-413.
Guo, Y., Chen, Y., Tan, Z. R., Klaassen, C. D., and Zhou, H. H. Repeated administration of berberine inhibits cytochromes P450 in humans. Eur J Clin Pharmacol 2012;68(2):213-217. View abstract.
Hajnicka, V., Kost'alova, D., Svecova, D., Sochorova, R., Fuchsberger, N., and Toth, J. Effect of Mahonia aquifolium active compounds on interleukin-8 production in the human monocytic cell line THP-1. Planta Med 2002;68(3):266-268. View abstract.
Hayasaka, S., Kodama, T., and Ohira, A. Traditional Japanese herbal (kampo) medicines and treatment of ocular diseases: a review. Am J Chin Med 2012;40(5):887-904. View abstract.
Hermann, R. and von, Richter O. Clinical evidence of herbal drugs as perpetrators of pharmacokinetic drug interactions. Planta Med 2012;78(13):1458-1477. View abstract.
Holick, M. F., Lamb, J. J., Lerman, R. H., Konda, V. R., Darland, G., Minich, D. M., Desai, A., Chen, T. C., Austin, M., Kornberg, J., Chang, J. L., Hsi, A., Bland, J. S., and Tripp, M. L. Hop rho iso-alpha acids, berberine, vitamin D3 and vitamin K1 favorably impact biomarkers of bone turnover in postmenopausal women in a 14-week trial. J Bone Miner.Metab 2010;28(3):342-350. View abstract.
Hong, Y., Hui, S. S., Chan, B. T., and Hou, J. Effect of berberine on catecholamine levels in rats with experimental cardiac hypertrophy. Life Sci. 4-18-2003;72(22):2499-2507. View abstract.
Hu, F. L. [Comparison of acid and Helicobacter pylori in ulcerogenesis of duodenal ulcer disease]. Zhonghua Yi.Xue.Za Zhi. 1993;73(4):217-9, 253. View abstract.
Hu, J. P., Takahashi, N., and Yamada, T. Coptidis rhizoma inhibits growth and proteases of oral bacteria. Oral Dis. 2000;6(5):297-302. View abstract.
Hu, Y., Ehli, E. A., Kittelsrud, J., Ronan, P. J., Munger, K., Downey, T., Bohlen, K., Callahan, L., Munson, V., Jahnke, M., Marshall, L. L., Nelson, K., Huizenga, P., Hansen, R., Soundy, T. J., and Davies, G. E. Lipid-lowering effect of berberine in human subjects and rats. Phytomedicine. 7-15-2012;19(10):861-867. View abstract.
Huang, W. [Ventricular tachyarrhythmias treated with berberine]. Zhonghua Xin.Xue.Guan.Bing.Za Zhi. 1990;18(3):155-6, 190. View abstract.
Huang, W. M., Wu, Z. D., and Gan, Y. Q. [Effects of berberine on ischemic ventricular arrhythmia]. Zhonghua Xin.Xue.Guan.Bing.Za Zhi. 1989;17(5):300-1, 319. View abstract.
Hui, K. K., Yu, J. L., Chan, W. F., and Tse, E. Interaction of berberine with human platelet alpha 2 adrenoceptors. Life Sci. 1991;49(4):315-324. View abstract.
Iizuka, N., Miyamoto, K., Okita, K., Tangoku, A., Hayashi, H., Yosino, S., Abe, T., Morioka, T., Hazama, S., and Oka, M. Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Lett 1-1-2000;148(1):19-25. View abstract.
Iizuka, N., Oka, M., Yamamoto, K., Tangoku, A., Miyamoto, K., Miyamoto, T., Uchimura, S., Hamamoto, Y., and Okita, K. Identification of common or distinct genes related to antitumor activities of a medicinal herb and its major component by oligonucleotide microarray. Int J Cancer 11-20-2003;107(4):666-672. View abstract.
Inbaraj, J. J., Kukielczak, B. M., Bilski, P., Sandvik, S. L., and Chignell, C. F. Photochemistry and photocytotoxicity of alkaloids from Goldenseal (Hydrastis canadensis L.) 1. Berberine. Chem Res Toxicol 2001;14(11):1529-1534. View abstract.
Inoue, K., Kulsum, U., Chowdhury, S. A., Fujisawa, S., Ishihara, M., Yokoe, I., and Sakagami, H. Tumor-specific cytotoxicity and apoptosis-inducing activity of berberines. Anticancer Res 2005;25(6B):4053-4059. View abstract.
Jantova, S., Cipak, L., Cernakova, M., and Kost'alova, D. Effect of berberine on proliferation, cell cycle and apoptosis in HeLa and L1210 cells. J Pharm Pharmacol 2003;55(8):1143-1149. View abstract.
Jeong, H. W., Hsu, K. C., Lee, J. W., Ham, M., Huh, J. Y., Shin, H. J., Kim, W. S., and Kim, J. B. Berberine suppresses proinflammatory responses through AMPK activation in macrophages. Am J Physiol Endocrinol.Metab 2009;296(4):E955-E964. View abstract.
Kamat SA. Clinical trials with berberine hydrochloride for the control of diarrhea in acute gastroenteritis. J Assoc Physicians India 1967;15:525-529.
Kaneda Y, Tanaka T, and Saw T. Effects of berberine, a plant alkaloid, on the growth of anaerobic protozoa in axenic culture. Tokai J Exp Clin Med 1990;15(6):417-423.
Kaneda Y, Torii M, Tanaka T, and et al. In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis. Annals of Tropical Medicine and Parasitology 1991;85(4):417-425.
Khin, Maung U. and Nwe, Nwe Wai. Effect of berberine on enterotoxin-induced intestinal fluid accumulation in rats. J Diarrhoeal Dis Res 1992;10(4):201-204. View abstract.
Khin, Maung U., Myo, Khin, Nyunt, Nyunt Wai, and Tin, U. Clinical trial of high-dose berberine and tetracycline in cholera. J Diarrhoeal Dis Res 1987;5(3):184-187. View abstract.
Khin, Maung U., Myo, Khin, Nyunt, Nyunt Wai, Aye, Kyaw, and Tin, U. Clinical trial of berberine in acute watery diarrhoea. Br.Med.J.(Clin.Res.Ed) 12-7-1985;291(6509):1601-1605. View abstract.
Kim, H. S., Kim, M. J., Kim, E. J., Yang, Y., Lee, M. S., and Lim, J. S. Berberine-induced AMPK activation inhibits the metastatic potential of melanoma cells via reduction of ERK activity and COX-2 protein expression. Biochem.Pharmacol 2-1-2012;83(3):385-394. View abstract.
Kim, W. S., Lee, Y. S., Cha, S. H., Jeong, H. W., Choe, S. S., Lee, M. R., Oh, G. T., Park, H. S., Lee, K. U., Lane, M. D., and Kim, J. B. Berberine improves lipid dysregulation in obesity by controlling central and peripheral AMPK activity. Am J Physiol Endocrinol.Metab 2009;296(4):E812-E819. View abstract.
Kong, W., Wei, J., Abidi, P., Lin, M., Inaba, S., Li, C., Wang, Y., Wang, Z., Si, S., Pan, H., Wang, S., Wu, J., Wang, Y., Li, Z., Liu, J., and Jiang, J. D. Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nat Med 2004;10(12):1344-1351. View abstract.
Krol R, Zalewski A, and Maroko PR. Beneficial effects of berberine, a new positive inotropic agent, on digitalis-induced ventricular arrhythmias. Circulation 1982;66(suppl 2):56.
Ksiezycka E, Cheung W, and Maroko PR. Antiarrhythmic effects of berberine on aconitine-induced ventricular and supraventricular arrhythmias. Clinical Research 1983;31(2):197A.
Kulkarni, S. K., Dandiya, P. C., and Varandani, N. L. Pharmacological investigations of berberine sulphate. Jpn.J Pharmacol. 1972;22(1):11-16. View abstract.
Kuo, C. L., Chi, C. W., and Liu, T. Y. Modulation of apoptosis by berberine through inhibition of cyclooxygenase-2 and Mcl-1 expression in oral cancer cells. In Vivo 2005;19(1):247-252. View abstract.
Kuo, C. L., Chou, C. C., and Yung, B. Y. Berberine complexes with DNA in the berberine-induced apoptosis in human leukemic HL-60 cells. Cancer Lett 7-13-1995;93(2):193-200. View abstract.
Lahiri S and Dutta NK. Berberine and chloramphenicol in the treatment of cholera and severe diarrhoea. Journal of the Indian Medical Association 1967;48(1):1-11.
Lamb, J. J., Holick, M. F., Lerman, R. H., Konda, V. R., Minich, D. M., Desai, A., Chen, T. C., Austin, M., Kornberg, J., Chang, J. L., Hsi, A., Bland, J. S., and Tripp, M. L. Nutritional supplementation of hop rho iso-alpha acids, berberine, vitamin D(3), and vitamin K(1) produces a favorable bone biomarker profile supporting healthy bone metabolism in postmenopausal women with metabolic syndrome. Nutr Res 2011;31(5):347-355. View abstract.
Lau, C. W., Yao, X. Q., Chen, Z. Y., Ko, W. H., and Huang, Y. Cardiovascular actions of berberine. Cardiovasc Drug Rev 2001;19(3):234-244. View abstract.
Lee, S., Lim, H. J., Park, H. Y., Lee, K. S., Park, J. H., and Jang, Y. Berberine inhibits rat vascular smooth muscle cell proliferation and migration in vitro and improves neointima formation after balloon injury in vivo. Berberine improves neointima formation in a rat model. Atherosclerosis 2006;186(1):29-37. View abstract.
Lee, Y. S., Kim, W. S., Kim, K. H., Yoon, M. J., Cho, H. J., Shen, Y., Ye, J. M., Lee, C. H., Oh, W. K., Kim, C. T., Hohnen-Behrens, C., Gosby, A., Kraegen, E. W., James, D. E., and Kim, J. B. Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states. Diabetes 2006;55(8):2256-2264. View abstract.
Li XB. [Controlled clinical trial in infants and children comparing Lacteol Fort sachets with two antidiarrhoeal reference drugs]. Ann Pediatr 1995;42(6):396-401.
Li, G. H., Wang, D. L., Hu, Y. D., Pu, P., Li, D. Z., Wang, W. D., Zhu, B., Hao, P., Wang, J., Xu, X. Q., Wan, J. Q., Zhou, Y. B., and Chen, Z. T. Berberine inhibits acute radiation intestinal syndrome in human with abdomen radiotherapy. Med Oncol. 2010;27(3):919-925. View abstract.
Li, H., Miyahara, T., Tezuka, Y., Namba, T., Suzuki, T., Dowaki, R., Watanabe, M., Nemoto, N., Tonami, S., Seto, H., and Kadota, S. The effect of kampo formulae on bone resorption in vitro and in vivo. II. Detailed study of berberine. Biol Pharm Bull 1999;22(4):391-396. View abstract.
Lin, C. C., Kao, S. T., Chen, G. W., Ho, H. C., and Chung, J. G. Apoptosis of human leukemia HL-60 cells and murine leukemia WEHI-3 cells induced by berberine through the activation of caspase-3. Anticancer Res 2006;26(1A):227-242. View abstract.
Lin, J. G., Chung, J. G., Wu, L. T., Chen, G. W., Chang, H. L., and Wang, T. F. Effects of berberine on arylamine N-acetyltransferase activity in human colon tumor cells. Am J Chin Med 1999;27(2):265-275. View abstract.
Lin, J. P., Yang, J. S., Lee, J. H., Hsieh, W. T., and Chung, J. G. Berberine induces cell cycle arrest and apoptosis in human gastric carcinoma SNU-5 cell line. World J Gastroenterol. 1-7-2006;12(1):21-28. View abstract.
Lin, S., Tsai, S. C., Lee, C. C., Wang, B. W., Liou, J. Y., and Shyu, K. G. Berberine inhibits HIF-1alpha expression via enhanced proteolysis. Mol Pharmacol 2004;66(3):612-619. View abstract.
Liu, Y., Yu, H., Zhang, C., Cheng, Y., Hu, L., Meng, X., and Zhao, Y. Protective effects of berberine on radiation-induced lung injury via intercellular adhesion molecular-1 and transforming growth factor-beta-1 in patients with lung cancer. Eur J Cancer 2008;44(16):2425-2432. View abstract.
Lu, S. S., Yu, Y. L., Zhu, H. J., Liu, X. D., Liu, L., Liu, Y. W., Wang, P., Xie, L., and Wang, G. J. Berberine promotes glucagon-like peptide-1 (7-36) amide secretion in streptozotocin-induced diabetic rats. J Endocrinol. 2009;200(2):159-165. View abstract.
Mahajan, V. M., Sharma, A., and Rattan, A. Antimycotic activity of berberine sulphate: an alkaloid from an Indian medicinal herb. Sabouraudia. 1982;20(1):79-81. View abstract.
Mantena, S. K., Sharma, S. D., and Katiyar, S. K. Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells. Mol Cancer Ther 2006;5(2):296-308. View abstract.
Marazzi, G., Cacciotti, L., Pelliccia, F., Iaia, L., Volterrani, M., Caminiti, G., Sposato, B., Massaro, R., Grieco, F., and Rosano, G. Long-term effects of nutraceuticals (berberine, red yeast rice, policosanol) in elderly hypercholesterolemic patients. Adv.Ther 2011;28(12):1105-1113. View abstract.
Marin-Neto, J. A., Maciel, B. C., Secches, A. L., and Gallo, Junior L. Cardiovascular effects of berberine in patients with severe congestive heart failure. Clin.Cardiol. 1988;11(4):253-260. View abstract.
Meng, S., Wang, L. S., Huang, Z. Q., Zhou, Q., Sun, Y. G., Cao, J. T., Li, Y. G., and Wang, C. Q. Berberine ameliorates inflammation in patients with acute coronary syndrome following percutaneous coronary intervention. Clin Exp.Pharmacol Physiol 2012;39(5):406-411. View abstract.
Mitani, N., Murakami, K., Yamaura, T., Ikeda, T., and Saiki, I. Inhibitory effect of berberine on the mediastinal lymph node metastasis produced by orthotopic implantation of Lewis lung carcinoma. Cancer Lett. 4-10-2001;165(1):35-42. View abstract.
Miyazaki, H., Shirai, E., Ishibashi, M., Hosoi, K., Shibata, S., and Iwanaga, M. Quantitation of berberine chloride in human urine by use of selected ion monitoring in the field desorption mode. Biomed.Mass Spectrom. 1978;5(10):559-565. View abstract.
Mohan, M., Pant, C. R., Angra, S. K., and Mahajan, V. M. Berberine in trachoma. (A clinical trial). Indian J Ophthalmol. 1982;30(2):69-75. View abstract.
Muller, K., Ziereis, K., and Gawlik, I. The antipsoriatic Mahonia aquifolium and its active constituents; II. Antiproliferative activity against cell growth of human keratinocytes. Planta Med 1995;61(1):74-75. View abstract.
Ni, Y. X. [Therapeutic effect of berberine on 60 patients with type II diabetes mellitus and experimental research]. Zhong.Xi.Yi.Jie.He.Za Zhi.- Chinese Journal of Modern Developments in Traditional Medicine 1988;8(12):711-3, 707. View abstract.
Ni, Y. X., Yang, J., and Fan, S. [Clinical study on jiang tang san in treating non-insulin dependent diabetes mellitus patients]. Zhongguo Zhong.Xi.Yi.Jie.He.Za Zhi. 1994;14(11):650-652. View abstract.
Nishida, S., Kikuichi, S., Yoshioka, S., Tsubaki, M., Fujii, Y., Matsuda, H., Kubo, M., and Irimajiri, K. Induction of apoptosis in HL-60 cells treated with medicinal herbs. Am J Chin Med 2003;31(4):551-562. View abstract.
Ozaki, Y., Suzuki, H., and Satake, M. [Comparative studies on concentration of berberine in plasma after oral administration of coptidis rhizoma extract, its cultured cells extract, and combined use of these extracts and glycyrrhizae radix extract in rats]. Yakugaku Zasshi 1993;113(1):63-69. View abstract.
Palasuntheram C, Iyer KS, de Silva LB, and et al. Antibacterial activity of Coscinium fenestratum Colebr against Clostridium tetani. Ind J Med Res 1982;76(Suppl):71-76.
Pan, J. F., Yu, C., Zhu, D. Y., Zhang, H., Zeng, J. F., Jiang, S. H., and Ren, J. Y. Identification of three sulfate-conjugated metabolites of berberine chloride in healthy volunteers' urine after oral administration. Acta Pharmacol Sin. 2002;23(1):77-82. View abstract.
Peng, W. H., Hsieh, M. T., and Wu, C. R. Effect of long-term administration of berberine on scopolamine-induced amnesia in rats. Jpn J Pharmacol 1997;74(3):261-266. View abstract.
Pisciotta, L., Bellocchio, A., and Bertolini, S. Nutraceutical pill containing berberine versus ezetimibe on plasma lipid pattern in hypercholesterolemic subjects and its additive effect in patients with familial hypercholesterolemia on stable cholesterol-lowering treatment. Lipids Health Dis 2012;11:123. View abstract.
Purohit SK, Kochar DK, Lal BB, and et al. Cultivation of Leishmania tropica from untreated and treated cases of oriental sore. Indian Journal of Public Health 1982;26(1):34-37.
Rabbani G. Mechanism and treatment of diarrhoea due to Vibrio cholerae and Escherichia coli: roles of drugs and prostaglandins. Danish Medical Bulletin 1996;43:173-185.
Sabir M and Bhide NK. Study of some pharmacological actions of berberine. Ind J Physiol & Pharmac 1971;15(3):111-132.
Sabir M, Mahajan VM, Mohapatra LN, and et al. Experimental study of the antitrachoma action of berberine. Indian J Med Res 1976;64(8):1160-1167.
Sack, R. B. and Froehlich, J. L. Berberine inhibits intestinal secretory response of Vibrio cholerae and Escherichia coli enterotoxins. Infect Immun. 1982;35(2):471-475. View abstract.
Saksena HC, Tomar VN, and Soangra MR. Efficacy of a new salt of Berberine Uni-Berberine in oriental sore. Current Medical Practice 1970;14:247-252.
Seery TM and Bieter RN. A contribution to the pharmacology of berberine. J Pharmacol Exp Ther 1940;69:64-67.
Seow WK, Ferrante A, Summors A, and et al. Comparative effects of tetrandrine and berbamine on production of the inflammatory cytokines interleukin-1 and tumor necrosis factor. Life Sciences 1992;50(8):pl-53-pl-58.
Shaffer, J. E. Inotropic and chronotropic activity of berberine on isolated guinea pig atria. J Cardiovasc Pharmacol 1985;7(2):307-315. View abstract.
Shanbhag, S. M., Kulkarni, H. J., and Gaitonde, B. B. Pharmacological actions of berberine on the central nervous system. Jpn.J Pharmacol 1970;20(4):482-487. View abstract.
Sharda DC. Berberine in the treatment of diarrhoea of infancy and childhood. J Indian M A 1970;54(1):22-24.
Sharma R, Joshi CK, and Goyal RK. Berberine tannate in acute diarrhoea. Indian Pediatrics 1970;7(9):496-501.
Soffar, S. A., Metwali, D. M., Abdel-Aziz, S. S., el Wakil, H. S., and Saad, G. A. Evaluation of the effect of a plant alkaloid (berberine derived from Berberis aristata) on Trichomonas vaginalis in vitro. J Egypt.Soc Parasitol. 2001;31(3):893-904. View abstract.
Sriwilaijareon, N., Petmitr, S., Mutirangura, A., Ponglikitmongkol, M., and Wilairat, P. Stage specificity of Plasmodium falciparum telomerase and its inhibition by berberine. Parasitol.Int 2002;51(1):99-103. View abstract.
Subbaiah TV and Amin AH. Effect of berberine sulphate on Entamoeba histolytica. Nature 1967;215(100):527-528.
Sun D, Courtney HS, and Beachey EH. Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane. Antimicrobial Agents and Chemotherapy 1988;32(9):1370-1374.
Supek Z and Tomic D. Farmakološko-kemijsko istrazivanje zutike (
Swabb, E. A., Tai, Y. H., and Jordan, L. Reversal of cholera toxin-induced secretion in rat ileum by luminal berberine. Am J Physiol 1981;241(3):G248-G252. View abstract.
Tai, Y. H., Feser, J. F., Marnane, W. G., and Desjeux, J. F. Antisecretory effects of berberine in rat ileum. Am J Physiol 1981;241(3):G253-G258. View abstract.
Thumm, H. W. and Tritschler, J. [The action of Berberin-drops on the intraocular pressure (IOP) (author's transl)]. Klin.Monbl.Augenheilkd. 1977;170(1):119-123. View abstract.
Trimarco, V., Cimmino, C. S., Santoro, M., Pagnano, G., Manzi, M. V., Piglia, A., Giudice, C. A., De, Luca N., and Izzo, R. Nutraceuticals for blood pressure control in patients with high-normal or grade 1 hypertension. High Blood Press Cardiovasc.Prev. 9-1-2012;19(3):117-122. View abstract.
Tripathi YB and Shukla SD. Berberis artistata inhibits PAF induced aggregation of rabbit platelets. Phytotherapy Research 1996;10:628-630.
Vik-Mo H, Faria DB, Cheung WM, and et al. Beneficial effects of berberine on left ventricular function in dogs with heart failure. Clinical Research 1983;31(2):224a.
Wang, D. Y., Yeh, C. C., Lee, J. H., Hung, C. F., and Chung, J. G. Berberine inhibited arylamine N-acetyltransferase activity and gene expression and DNA adduct formation in human malignant astrocytoma (G9T/VGH) and brain glioblastoma multiforms (GBM 8401) cells. Neurochem.Res 2002;27(9):883-889. View abstract.
Wang, N., Feng, Y., Cheung, F., Chow, O. Y., Wang, X., Su, W., and Tong, Y. A comparative study on the hepatoprotective action of bear bile and Coptidis Rhizoma aqueous extract on experimental liver fibrosis in rats. BMC.Complement Altern.Med 2012;12:239. View abstract.
Wang, Q., Zhang, M., Liang, B., Shirwany, N., Zhu, Y., and Zou, M. H. Activation of AMP-activated protein kinase is required for berberine-induced reduction of atherosclerosis in mice: the role of uncoupling protein 2. PLoS.One. 2011;6(9):e25436. View abstract.
Wang, Y., Jia, X., Ghanam, K., Beaurepaire, C., Zidichouski, J., and Miller, L. Berberine and plant stanols synergistically inhibit cholesterol absorption in hamsters. Atherosclerosis 2010;209(1):111-117. View abstract.
Wei, W., Zhao, H., Wang, A., Sui, M., Liang, K., Deng, H., Ma, Y., Zhang, Y., Zhang, H., and Guan, Y. A clinical study on the short-term effect of berberine in comparison to metformin on the metabolic characteristics of women with polycystic ovary syndrome. Eur J Endocrinol. 2012;166(1):99-105. View abstract.
Wright, C. W., Marshall, S. J., Russell, P. F., Anderson, M. M., Phillipson, J. D., Kirby, G. C., Warhurst, D. C., and Schiff, P. L. In vitro antiplasmodial, antiamoebic, and cytotoxic activities of some monomeric isoquinoline alkaloids. J Nat Prod 2000;63(12):1638-1640. View abstract.
Wu, H. L., Hsu, C. Y., Liu, W. H., and Yung, B. Y. Berberine-induced apoptosis of human leukemia HL-60 cells is associated with down-regulation of nucleophosmin/B23 and telomerase activity. Int J Cancer 6-11-1999;81(6):923-929. View abstract.
Wu, J. F. and Liu, T. P. [Effects of berberine on platelet aggregation and plasma levels of TXB2 and 6-keto-PGF1 alpha in rats with reversible middle cerebral artery occlusion]. Yao Xue.Xue.Bao. 1995;30(2):98-102. View abstract.
Wu, S. N., Yu, H. S., Jan, C. R., Li, H. F., and Yu, C. L. Inhibitory effects of berberine on voltage- and calcium-activated potassium currents in human myeloma cells. Life Sci 1998;62(25):2283-2294. View abstract.
Xin, H. W., Wu, X. C., Li, Q., Yu, A. R., Zhong, M. Y., and Liu, Y. Y. The effects of berberine on the pharmacokinetics of cyclosporin A in healthy volunteers. Methods Find.Exp.Clin Pharmacol 2006;28(1):25-29. View abstract.
Xu, M. G., Wang, J. M., Chen, L., Wang, Y., Yang, Z., and Tao, J. Berberine-induced mobilization of circulating endothelial progenitor cells improves human small artery elasticity. J Hum.Hypertens 2008;22(6):389-393. View abstract.
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Yang, Z., Shao, Y. C., Li, S. J., Qi, J. L., Zhang, M. J., Hao, W., and Jin, G. Z. Medication of l-tetrahydropalmatine significantly ameliorates opiate craving and increases the abstinence rate in heroin users: a pilot study. Acta Pharmacol Sin. 2008;29(7):781-788. View abstract.
Yin, J., Gao, Z., Liu, D., Liu, Z., and Ye, J. Berberine improves glucose metabolism through induction of glycolysis. Am J Physiol Endocrinol.Metab 2008;294(1):E148-E156. View abstract.
Yin, J., Xing, H., and Ye, J. Efficacy of berberine in patients with type 2 diabetes mellitus. Metabolism 2008;57(5):712-717. View abstract.
Yount, G., Qian, Y., Moore, D., Basila, D., West, J., Aldape, K., Arvold, N., Shalev, N., and Haas-Kogan, D. Berberine sensitizes human glioma cells, but not normal glial cells, to ionizing radiation in vitro. J Exp Ther Oncol. 2004;4(2):137-143. View abstract.
Yuan, J., Shen, X. Z., and Zhu, X. S. [Effect of berberine on transit time of human small intestine]. Zhongguo Zhong.Xi.Yi.Jie.He.Za Zhi. 1994;14(12):718-720. View abstract.
Zalewski A, Krol R, and Maroko PR. Berberine, a new inotropic agent - distinction between its cardiac and peripheral responses. Clin Res 1983;31(2):227A.
Zeng, X. and Zeng, X. Relationship between the clinical effects of berberine on severe congestive heart failure and its concentration in plasma studied by HPLC. Biomed Chromatogr 1999;13(7):442-444. View abstract.
Zhang, H., Wei, J., Xue, R., Wu, J. D., Zhao, W., Wang, Z. Z., Wang, S. K., Zhou, Z. X., Song, D. Q., Wang, Y. M., Pan, H. N., Kong, W. J., and Jiang, J. D. Berberine lowers blood glucose in type 2 diabetes mellitus patients through increasing insulin receptor expression. Metabolism 2010;59(2):285-292. View abstract.
Zhang, M. F. and Shen, Y. Q. [Antidiarrheal and anti-inflammatory effects of berberine]. Zhongguo Yao Li Xue.Bao. 1989;10(2):174-176. View abstract.
Zhang, Y., Li, X., Zou, D., Liu, W., Yang, J., Zhu, N., Huo, L., Wang, M., Hong, J., Wu, P., Ren, G., and Ning, G. Treatment of type 2 diabetes and dyslipidemia with the natural plant alkaloid berberine. J Clin Endocrinol.Metab 2008;93(7):2559-2565. View abstract.
Zhou, J. Y., Zhou, S. W., Zhang, K. B., Tang, J. L., Guang, L. X., Ying, Y., Xu, Y., Zhang, L., and Li, D. D. Chronic effects of berberine on blood, liver glucolipid metabolism and liver PPARs expression in diabetic hyperlipidemic rats. Biol Pharm Bull. 2008;31(6):1169-1176. View abstract.
Zhu B and Ahrens FA. Effect of berberine on intestinal secretion mediated by Escherichia coli heat-stable enterotoxin in jejunum of pigs. Am J Vet Res 1982;43(9):1594-1598.
Zhu, B. and Ahrens, F. Antisecretory effects of berberine with morphine, clonidine, L- phenylephrine, yohimbine or neostigmine in pig jejunum. Eur J Pharmacol 12-9-1983;96(1-2):11-19. View abstract.
Coughlan KA, Valentine RJ, Ruderman NB, Saha AK. AMPK activation: a therapeutic target for type 2 diabetes? Diabetes Metab Syndr Obes 2014;7:241-53. View abstract.
Abascal K, Yarnell E. Recent clinical advances with berberine. Altern Complement Ther 2010;16(5):281-7.
Amin AH, Subbaiah TV, Abbasi KM. Berberine sulfate: antimicrobial activity, bioassay, and mode of action. Can J Microbiol 1969;15:1067-76. View abstract.
An Y, Sun Z, Zhang Y, Liu B, Guan Y, Lu M. The use of berberine for women with polycystic ovary syndrome undergoing IVF treatment. Clin Endocrinol (Oxf) 2014;80(3):425-31. View abstract.
Ang ES, Lee ST, Gan CS, et al. Evaluating the role of alternative therapy in burn wound management: randomized trial comparing moist exposed burn ointment with conventional methods in the management of patients with second-degree burns. MedGenMed 2001;3:3. View abstract.
Anis KV, Rajeshkumar NV, Kuttan R. Inhibition of chemical carcinogenesis by berberine in rats and mice. J Pharm Pharmacol 2001;53:763-8. . View abstract.
Asbaghi O, Ghanbari N, Shekari M, et al. The effect of berberine supplementation on obesity parameters, inflammation and liver function enzymes: a systematic review and meta-analysis of randomized controlled trials. Clin Nutr ESPEN 2020;38:43-9. View abstract.
Beba M, Djafarian K, Shab-Bidar S. Effect of Berberine on C-reactive protein: a systematic review and meta-analysis of randomized controlled trials. Complement Ther Med. 2019;46:81-6. View abstract.
Bhide MB, Chavan SR, Dutta NK. Absorption, distribution and excretion of berberine. Indian J Med Res 1969;57:2128-31. View abstract.
Blais JE, Huang X, Zhao JV. Overall and Sex-Specific Effect of Berberine for the Treatment of Dyslipidemia in Adults: A Systematic Review and Meta-Analysis of Randomized Placebo-Controlled Trials. Drugs 2023;83(5):403-427. View abstract.
Budzinski JW, Foster BC, Vandenhoek S, Arnason JT. An in vitro evaluation of human cytochrome P450 3A4 inhibition by selected commercial herbal extracts and tinctures. Phytomedicine 2000;7:273-82. View abstract.
Butcher NJ, Minchin RF. Arylamine N-acetyltransferase 1: a novel drug target in cancer development. Pharmacol Rev 2012;64(1):147-65. View abstract.
Chan E. Displacement of bilirubin from albumin by berberine. Biol Neonate 1993;63:201-8. View abstract.
Chan M, Qin Z, Man SC, et al. Adjunctive berberine reduces antipsychotic-associated weight gain and metabolic syndrome in patients with schizophrenia: a randomized controlled trial. Psychiatry Clin Neurosci 2022;76(3):77-85. View abstract.
Chatterjee P, Franklin MR. Human cytochrome p450 inhibition and metabolic-intermediate complex formation by goldenseal extract and its methylenedioxyphenyl components. Drug Metab Dispos 2003;31:1391-7. View abstract.
Chen C, Tao C, Liu Z, Lu M, Pan Q, Zheng L, et al. A randomized clinical trial of berberine hydrochloride in patients with diarrhea-predominant irritable bowel syndrome. Phytother Res. 2015 Nov;29(11):1822-7. doi: 10.1002/ptr.5475. View abstract.
Chen S, Shen W, Liu Y, Dong Q, Shi Y. Efficacy and safety of triple therapy containing berberine, amoxicillin, and vonoprazan for Helicobacter pylori initial treatment: A randomized controlled trial. Chin Med J (Engl) 2023;136(14):1690-1698. View abstract.
Chen XX, Chen YX, Bi HX, et al. Efficacy and safety of triple therapy containing berberine hydrochloride, amoxicillin, and rabeprazole in the eradication of Helicobacter pylori. J Dig Dis 2022;23(10):568-76. View abstract.
Chen YX, Gao QY, Zou TH, et al. Berberine versus placebo for the prevention of recurrence of colorectal adenoma: a multicentre, double-blinded, randomised controlled study. Lancet Gastroenterol Hepatol. 2020;5(3):267-75. View abstract.
Cicero, AF, Rovati LC, and Setnikar I. Eulipidemic effects of berberine administered alone or in combination with other natural cholesterol-lowering agents. A single-blind clinical investigation. Arzneimittelforschung. 2007;57:26-30. View abstract.
Déléaval M, Burri H, Bakelants E. Harmless herbs? A case report of acquired long QT syndrome and torsades de pointes in a patient taking herbal supplements. HeartRhythm Case Rep 2022;8(5):309-12. View abstract.
Dong H, Zhao Y, Zhao L, Lu F. The effects of berberine on blood lipids: a systemic review and meta-analysis of randomized controlled trials. Planta Med 2013;79(6):437-46. View abstract.
Fang S, Guo S, Du S, et al. Efficacy and safety of berberine in preventing recurrence of colorectal adenomas: A systematic review and meta-analysis. J Ethnopharmacol 2022;282:114617. View abstract.
Fukuda K, Hibiya Y, Mutoh M, et al. Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells. J Ethnopharmacol 1999;66:227-33. View abstract.
Garber AJ. Long-acting glucagon-like peptide 1 receptor agonists: a review of their efficacy and tolerability. Diabetes Care 2011;34 Suppl 2:S279-84. View abstract.
Guo J, Chen H, Zhang X, et al. The effect of berberine on metabolic profiles in type 2 diabetic patients: a systematic review and meta-analysis of randomized controlled trials. Oxid Med Cell Longev 2021;2021:2074610. View abstract.
Gupte S. Use of berberine in treatment of giardiasis. Am J Dis Child 1975;129:866. View abstract.
Hou Q, Han W, Fu X. Pharmacokinetic interaction between tacrolimus and berberine in a child with idiopathic nephrotic syndrome. Eur J Clin Pharmacol 2013;69(10):1861-2. View abstract.
Hsiang CY, Wu SL, Cheng SE, Ho TY. Acetaldehyde-induced interleukin-1beta and tumor necrosis factor-alpha production is inhibited by berberine through nuclear factor-kappaB signaling pathway in HepG2 cells. J Biomed Sci 2005;12:791-801. View abstract.
Huang CG, Chu ZL, Wei SJ, Jiang H, Jiao BH. Effect of berberine on arachidonic acid metabolism in rabbit platelets and endothelial cells. Thromb Res 2002;106(4-5):223-7. View abstract.
Huang XS, Yang GF, Pan YC. Effect of berberin hydrochloride on blood concentration of cyclosporine A in cardiac transplanted patients. Zhongguo Zhong Xi Yi Jie He Za Zhi 2008;28:702-4. View abstract.
Ivanovska N, Philipov S. Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int J Immunopharmacol 1996;18:553-61. View abstract.
Janbaz KH, Gilani AH. Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia 2000;71:25-33.. View abstract.
Jiang XW, Zhang Y, Zhu YL, et al. Effects of berberine gelatin on recurrent aphthous stomatitis: a randomized, placebo-controlled, double-blind trial in a Chinese cohort. Oral Surg Oral Med Oral Pathol Oral Radiol 2013;115(2):212-7. View abstract.
Ju J, Li J, Lin Q, Xu H. Efficacy and safety of berberine for dyslipidaemias: A systematic review and meta-analysis of randomized clinical trials. Phytomedicine. 2018;50:25-34. View abstract.
Kaneda Y, Torii M, Tanaka T, Aikawa M. In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis. Ann Trop Med Parasitol 1991;85:417-25. View abstract.
Khosla PG, Neeraj VI, Gupta SK, et al. Berberine, a potential drug for trachoma. Rev Int Trach Pathol Ocul Trop Subtrop Sante Publique 1992;69:147-65. View abstract.
Kim JS, Tanaka H, Shoyama Y. Immunoquantitative analysis for berberine and its related compounds using monoclonal antibodies in herbal medicines. Analyst 2004;129:87-91. View abstract.
Kim SH, Shin DS, Oh MN, et al. Inhibition of the bacterial surface protein anchoring transpeptidase sortase by isoquinoline alkaloids. Biosci Biotechnol Biochem 2004;68:421-4.. View abstract.
Kowdley KV, Forman L, Eksteen B, et al. A randomized, dose-finding, proof-of-concept study of berberine ursodeoxycholate in patients with primary sclerosing cholangitis. Am J Gastroenterol 2022;117(11):1805-15. View abstract.
Lan J, Zhao Y, Dong F, et al. Meta-analysis of the effect and safety of berberine in the treatment of type 2 diabetes mellitus, hyperlipemia and hypertension. J Ethnopharmacol. 2015;161:69-81. View abstract.
Li B, Shang JC, Zhou QX. [Study of total alkaloids from rhizoma coptis chinensis on experimental gastric ulcers]. Chin J Integr Med 2005;11:217-21. View abstract.
Li G, Zhao M, Qiu F, Sun Y, Zhao L. Pharmacokinetic interactions and tolerability of berberine chloride with simvastatin and fenofibrate: an open-label, randomized, parallel study in healthy Chinese subjects. Drug Des Devel Ther. 2018;13:129-139. View abstract.
Lu Y, Zhang X, He J, et al. The effects of berberine on inflammatory markers in Chinese patients with metabolic syndrome and related disorders: a meta-analysis of randomized controlled trials. Inflammopharmacology 2022;30(3):1063-77. View abstract.
Luo D, Yu B, Sun S, et al. Effects of adjuvant berberine therapy on acute ischemic stroke: A meta-analysis. Phytother Res 2023;37(9):3820-3838. View abstract.
Lyu Y, Zhang Y, Yang M, et al. Pharmacokinetic interactions between metformin and berberine in rats: Role of oral administration sequences and microbiota. Life Sci. 2019;235:116818. View abstract.
Marazzi G, Campolongo G, Pelliccia F, Quattrino S, Vitale C, Cacciotti L, et al. Comparison of low-dose statin versus low-dose statin + Armolipid plus in high-intensity statin-intolerant patients with a previous coronary event and percutaneous coronary intervention (ADHERENCE trial). Am J Cardiol. 2017 Sep 15;120(6):893-897. doi: 10.1016/j.amjcard.2017.06.015. View abstract.
Marazzi G, Pelliccia F, Campolongo G, Quattrino S, Cacciotti L, Volterrani M, et al. Usefulness of nutraceuticals (Armolipid Plus) versus ezetimibe and combination in statin-intolerant patients with dyslipidemia with coronary heart disease. Am J Cardiol. 2015 Dec 15;116(12):1798-801. doi: 10.1016/j.amjcard.2015.09.023. View abstract.
Millán J, Cicero AF, Torres F, Anguera A. Effects of a nutraceutical combination containing berberine (BRB), policosanol, and red yeast rice (RYR), on lipid profile in hypercholesterolemic patients: A meta-analysis of randomized controlled trials. Clin Investig Arterioscler. 2016;28(4):178-87. View abstract.
Ming J, Yu X, Xu X, et al. Effectiveness and safety of Bifidobacterium and berberine in human hyperglycemia and their regulatory effect on the gut microbiota: a multi-center, double-blind, randomized, parallel-controlled study. Genome Med 2021;13(1):125. View abstract.
Moon JM, Ratliff KM, Hagele AM, Stecker RA, Mumford PW, Kerksick CM. Absorption kinetics of berberine and dihydroberberine and their impact on glycemia: a randomized, controlled, crossover pilot trial. Nutrients 2021;14(1):124. View abstract.
Panigrahi A, Mohanty S. Efficacy and safety of HIMABERB® Berberine on glycemic control in patients with prediabetes: double-blind, placebo-controlled, and randomized pilot trial. BMC Endocr Disord 2023;23(1):190. View abstract.
Park KS, Kang KC, Kim JH, et al. Differential inhibitory effects of protoberberines on sterol and chitin biosyntheses in Candida albicans. J Antimicrob Chemother 1999;43:667-74. View abstract.
Pérez-Rubio KG, González-Ortiz M, Martínez-Abundis E, Robles-Cervantes JA, Espinel-Bermúdez MC. Effect of berberine administration on metabolic syndrome, insulin sensitivity, and insulin secretion. Metab Syndr Relat Disord 2013;11(5):366-9. View abstract.
Qiao M, Lei C, Tan C, Lu C, Chen Z, Zhang Q, Wang Z. Efficacy and safety of berberine for premature ventricular contractions: a meta-analysis and systematic review of randomized controlled trials. Pharm Biol. 2023 Dec;61(1):1474-1483. View abstract.
Qing Y, Dong X, Hongli L, Yanhui L. Berberine promoted myocardial protection of postoperative patients through regulating myocardial autophagy. Biomed Pharmacother. 2018;105:1050-1053. View abstract.
Rabbani GH, Butler T, Knight J, et al. Randomized controlled trial of berberine sulfate therapy for diarrhea due to enterotoxigenic Escherichia coli and Vibrio cholerae. J Infect Dis 1987;155:979-84. View abstract.
Rehman J, Dillow JM, Carter SM, et al. Increased production of antigen-specific immunoglobulins G and M following in vivo treatment with the medicinal plants Echinacea angustifolia and Hydrastis canadensis. Immunol Lett 1999;68:391-5. View abstract.
Rondanelli M, Gasparri C, Petrangolini G, et al. Berberine phospholipid exerts a positive effect on the glycemic profile of overweight subjects with impaired fasting blood glucose (IFG): a randomized double-blind placebo-controlled clinical trial. Eur Rev Med Pharmacol Sci 2023;27(14):6718-6727. View abstract.
Rondanelli M, Riva A, Petrangolini G, et al. Berberine Phospholipid Is an Effective Insulin Sensitizer and Improves Metabolic and Hormonal Disorders in Women with Polycystic Ovary Syndrome: A One-Group Pretest-Post-Test Explanatory Study. Nutrients. 2021;13(10):3665. View abstract.
Ruscica M, Gomaraschi M, Mombelli G, Macchi C, Bosisio R, Pazzucconi F, Pavanello C, Calabresi L, Arnoldi A, Sirtori CR, Magni P. Nutraceutical approach to moderate cardiometabolic risk: results of a randomized, double-blind and crossover study with Armolipid Plus. J Clin Lipidol. 2014;8(1):61-8. View abstract.
Scazzocchio F, Corneta MF, Tomassini L, Palmery M. Antibacterial activity of Hydrastis canadensis extract and its major isolated alkaloids. Planta Med 2001;67:561-4. View abstract.
Sheng WD, Jiddawi MS, Hong XQ, Abdulla SM. Treatment of chloroquine-resistant malaria using pyrimethamine in combination with berberine, tetracycline, or cotrimoxazole. East Afr Med J 1997;74:283-4. View abstract.
Sun D, Abraham SN, Beachey EH. Influence of berberine sulfate on synthesis and expression of Pap fimbrial adhesin in uropathogenic Escherichia coli. Antimicrob Agents Chemother 1988;32:1274-7. View abstract.
Sun D, Courtney HS, Beachey EH. Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane. Antimicrob Agents Chemother 1988;32:1370-4. View abstract.
Tsai PL, Tsai TH. Hepatobiliary excretion of berberine. Drug Metab Dispos 2004;32:405-12. . View abstract.
Vollekova A, Kost'alova D, Kettmann V, Toth J. Antifungal activity of Mahonia aquifolium extract and its major protoberberine alkaloids. Phytother Res 2003;17:834-7. View abstract.
Wang S, Ren H, Zhong H, et al. Combined berberine and probiotic treatment as an effective regimen for improving postprandial hyperlipidemia in type 2 diabetes patients: a double blinded placebo controlled randomized study. Gut Microbes 2022;14(1):2003176. View abstract.
Wen C, Wu L, Fu L, Zhang X, Zhou H. Berberine enhances the anti tumor activity of tamoxifen in drug sensitive MCF 7 and drug resistant MCF 7/TAM cells. Mol Med Rep. 2016;14(3):2250-6. View abstract.
Wu X, Li Q, Xin H, Yu A, Zhong M. Effects of berberine on the blood concentration of cyclosporin A in renal transplanted recipients: clinical and pharmacokinetic study. Eur J Clin Pharmacol 2005;61:567-72. View abstract.
Wu XK, Wang YY, Liu JP, Liang RN, Xue HY, Ma HX, et al. Randomized controlled trial of letrozole, berberine, or a combination for infertility in the polycystic ovary syndrome. Fertil Steril. 2016;106(3):757-765.e1. doi: 10.1016/j.fertnstert.2016.05.022. View abstract.
Xie, X., Meng, X., Zhou, X., Shu, X., and Kong, H. [Research on therapeutic effect and hemorrheology change of berberine in new diagnosed patients with type 2 diabetes combining nonalcoholic fatty liver disease]. Zhongguo Zhong Yao Za Zhi 2011;36(21):3032-3035. View abstract.
Xu L, Zhang Y, Xue X, et al. A phase I trial of berberine in Chinese with ulcerative colitis. Cancer Prev Res (Phila). 2020;13(1):117-26. View abstract.
Yan HM, Xia MF, Wang Y, Chang XX, Yao XZ, Rao SX, et al. Efficacy of berberine in patients with non-alcoholic fatty liver disease. PLoS One. 2015 Aug 7;10(8):e0134172. doi: 10.1371/journal.pone.0134172. View abstract.
Yang L, Zhu W, Zhang X, Zhou X, Wu W, Shen T. Efficacy and safety of berberine for several cardiovascular diseases: A systematic review and meta-analysis of randomized controlled trials. Phytomedicine 2023;112:154716. View abstract.
Zeng XH, Zeng XJ, Li YY. Efficacy and safety of berberine for congestive heart failure secondary to ischemic or idiopathic dilated cardiomyopathy. Am J Cardiol 2003;92:173-6. View abstract.
Zhang D, Ke L, Ni Z, Chen Y, Zhang LH, Zhu SH, et al. Berberine containing quadruple therapy for initial Helicobacter pylori eradication: An open-label randomized phase IV trial. Medicine (Baltimore). 2017;96(32):e7697. doi: 10.1097/MD.0000000000007697. View abstract.
Zhang J, Wang Y, Jiang H, et al. Preventive effect of berberine on postoperative atrial fibrillation. Circ Arrhythm Electrophysiol 2022;15(10):e011160. View abstract.
Zhang LS, Zhang JH, Feng R, et al. Efficacy and safety of berberine alone or combined with statins for the treatment of hyperlipidemia: a systematic review and meta-analysis of randomized controlled clinical trials. Am J Chin Med 2019;47(4):751-67. View abstract.
Zhang Y, Li X, Zou D, et al. Treatment of type 2 diabetes and dyslipidemia with the natural plant alkaloid berberine. J Clin Endocrinol Metab 2008;93:2559-65. View abstract.
Berberine hydrochloride is a conventional component in Chinese medicine, and is characterized by a diversity of pharmacological effects. However, due to its hydrophobic properties, along with poor stability and bioavailability, the application of berberine hydrochloride was hampered for a long time. In recent years, the pharmaceutical preparation of berberine hydrochloride has improved to achieve good prospects for clinical application, especially for novel nanoparticulate delivery systems. Moreover, anticancer activity and novel mechanisms have been explored, the chance of regulating glucose and lipid metabolism in cancer cells showing more potential than ever. Therefore, it is expected that appropriate pharmaceutical procedures could be applied to the enormous potential for anticancer efficacy, to give some new insights into anticancer drug preparation in Chinese medicine.
We accessed conventional databases, such as PubMed, Scope, and Web of Science, using “berberine hydrochloride”, “anti-cancer mechanism”, and “nanoparticulate delivery system” as search words, then summarized the progress in research, illustrating the need to explore reprogramming of cancer cell metabolism using nanoparticulate drug delivery systems.
With increasing research on regulation of cancer cell metabolism by berberine hydrochloride and troubleshooting of issues concerning nanoparticulate delivery preparation, berberine hydrochloride is likely to become a natural component of the nanoparticulate delivery systems used for cancer therapy. Meanwhile, the known mechanisms of berberine hydrochloride, such as decreased multidrug resistance and enhanced sensitivity of chemotherapeutic drugs, along with improvement in patient quality of life, could also provide new insights into cancer cell metabolism and nanoparticulate delivery preparation.
Keywords:
berberine hydrochloride, anticancer mechanisms, nanoparticulate drug progress
Berberine hydrochloride is an isoquinoline alkaloid (see ) isolated from a variety of Chinese herbs, including Coptidis rhizoma, Phellodendron chinense schneid, and Phellodendron amurense, and has diverse pharmacological actions. It has antidiabetic and antilipid peroxidation activity, as well as an anti-atherosclerotic action, and also has neuroprotective properties and improves polycystic ovary syndrome.1–5 Berberine hydrochloride is widely used as an antibacterial, antifungal, and anti-inflammatory drug, and has been used as a gastrointestinal remedy for thousands of years in China.6,7
Open in a separate windowNowadays, the antiproliferative activity and sensitivity enhancement of berberine hydrochloride in various cancer cell lines8–14 have led to further research interest in this compound.15–17 Its antineoplastic properties include induction of apoptosis and cell cycle arrest, along with inhibition of cell migration and invasion via regulation of multiple pathways.18–21 The potential targets of berberine hydrochloride include reactive oxygen species generation, mitochondrial function, DNA topoisomerase inhibition, DNA or RNA binding, the estrogen receptor, matrix metalloproteinase regulation, p53 activation, and NF-kappa B signal activation.10,22–26 However, it has poor water solubility caused by a quarternary amine, resulting in a low effective concentration and limited absorption in the gastrointestinal tract, which seriously limits its application and development as a pharmaceutical preparation. Furthermore, it has a risk of adverse reactions associated with intramuscular and intravenous administration, such as anaphylactic shock and drug rash, so a novel drug delivery system to improve the solubility and bioavailability of berberine hydrochloride has become a matter of urgency.
During the rapid development of nanotechnology, increasing attention has been paid to nanoparticulate drug delivery systems.27–29 Modern nanoparticulate dosage forms including polymeric nanoparticles, nanocapsules, liposomes, solid lipid nanoparticles, and nanoemulsions, all of which can improve drug solubility. In general, nanoparticulate drug delivery enhances solubility and bioavailability, improving pharmacological activity and tissue macrophage distribution, while preventing physical and chemical degradation.30,31 Therefore, we combined the good anticancer efficacy of berberine hydrochloride with a novel nanoparticulate drug delivery system to obtain a promising anticancer agent.
This review discusses anticancer mechanisms, with particular reference to regulation of glucose and lipid metabolism, and describes a novel drug delivery system for berberine hydrochloride, aiming to provide new insights into Chinese medicine preparations with anticancer activity.
The potential antitumor activity of berberine hydrochloride has always been a subject of considerable interest because of its known ability to interact with nucleic acids. Its ability to bind specifically to oligonucleotides and to stabilize DNA triplexes or G-quadruplexes via telomerase and topoisomerase inhibition accounts for its antiproliferative activity.32,33 The predominant interaction between berberine hydrochloride and double-stranded or single-stranded DNA is electrostatic, and can be quantified in terms of the Hill model of cooperative interactions.34 Recent novel mechanisms have a higher propensity for autophagy and autophagic regulators. Wang et al found that berberine hydrochloride induced autophagic cell death which was diminished by 3-methyladenine, a cell death inhibitor, in the human hepatic carcinoma cell lines HepG2 and MHCC97-L, through activation of beclin-1 and inhibition of the mTOR signaling pathway.35 In addition, the autophagic marker, microtubule-associated protein-1 light chain 3 (LC3) was modified after administration of berberine hydrochloride in the human A549 lung cancer cell line, accompanied by shrinkage of tumor volume in a Lewis lung carcinoma model in mice, all of which indicates that autophagy might be important in cancer cell death.36
In addition to autophagy and its interaction with nucleic acid, the hypoglycemic and hypolipidemic effects of berberine hydrochloride also point to a relationship between adipose tissue/adipocytes and tumorigenesis, through upregulation of mRNA and protein levels in adipose tissue, including peroxisome proliferator-activated receptor (PPAR) α, β, and γ, CDK9, and cyclin T1.3 Adipose tissue and adipocytes have a significant role in the tumor microenvironment,37 and SPARC (secreted protein acidic and rich in cysteine), an adiposity inhibitor, was suggested by Nagaraju and Sharma38 to be a potent anticancer molecule, and human adipose tissue-derived stem cells are known to be a source of carcinoma-associated fibroblasts in the presence of transforming growth factor β1.39 Further, adipose tissue-derived vascular endothelial growth factor and leptin promote adipogenesis in order to maintain the tumor microenvironment.40 Hirano et al have also suggested the existence of undefined factors derived from cancer cells which promote adipogenesis, further indicating a potential relationship between adipogenesis and development of cancer.41 In clinical lipofilling procedures undertaken for patients with breast cancer, there is an urgent need to clarify the issue of cancer recurrence and adipogenesis.42 The adipogenesis positive regulator, PPARγ, overexpressed in ERBB2-positive breast cancer cells, enables fatty acid synthesis, mainly to support energy demands and cell survival.43 Therefore, less toxic PPARγ agonists or antagonists, including berberine hydrochloride, are regarded as potential agents for improving adipose breast tissue and decreasing breast cancer risk, as well as suppressing proliferation and invasion of cancer cells.44 By inhibiting PPARγ protein expression and increasing PPARα mRNA levels, berberine hydrochloride has been shown to improve free fatty acid-induced insulin resistance in myotubes, and to suppress adipogenesis in white preadipocytes in humans and hepatic insulin resistance in diabetic hamsters.2,45,46 Berberine hydrochloride also prevented wasting of epididymal adipose tissue and ameliorated cancer cachexia in colon 26/clone 20-transplanted mice and colon 26/clone 20 cells,47 further highlighting the beneficial effect of this compound on adipose tissue in the tumor microenvironment.
Working as a potential natural compound in cancer therapy via its interaction with nucleic acid and regulation of cancer cells, as well as induction of autophagy, berberine hydrochloride augments the effects of chemotherapy/radiotherapy and has shown good prospects in cancer treatment.11 After the novel mechanisms by which it interferes with the development of adipose tissue and adipocyte metabolism in the tumor microenvironment were investigated, the efficacy and potential applications of berberine hydrochloride were highlighted and emphasized. Moreover, its extensive occurrence in various plant species and low toxicity suggest that berberine hydrochloride has the potential to become an effective antitumor agent in the future.
Reports on nanoparticulate delivery systems for berberine hydrochloride can be divided into three types, ie, solid lipid nanoparticles, nanoemulsions, and liposomes. Preparation, characterization, experimental design methods, and in vivo and in vitro studies are summarized here.
The first nanoparticulate delivery system uses a conventional rotary-evaporated film-ultrasonication method to make solid lipid nanoparticles of berberine hydrochloride (BH-SLN). These have good stability, a mean diameter of 60.5 nm, a zeta potential of 29.7 mV, drug loading of 8.69%, and an entrapment ratio of 97.58%.48 Certain other factors have a direct impact on actual amount and quality of liposome entrapment, including preparation and manufacturing methods, and types of excipients used and particle size. Entrapment is defined as the fraction of the initial solution remaining within the liposomes, which is the key factor in clinical application.49 Wang et al established the method of coagulation centrifugation to determine the entrapment efficiency of BH-SLN.50 A saturated aqueous solution of sodium chloride 0.05 mL in BH-SLN 0.5 mL was determined by high-pressure liquid chromatography, and then centrifuged at 12,000 rpm for 10 minutes to obtain the supernatant. The results show that coagulation centrifugation was rapid and accurate.
The second delivery system is berberine hydrochloride nanoemulsion, made by isopropyl myristate, EL40, and glycerin using pseudoternary phase diagrams. The nanoemulsion is a clear transparent solution with an average particle diameter of 56.8 nm.51 Small spherical drops are seen under electron microscopy, with a stable content and diameter even under high humidity and temperature conditions, along with strong light, 92.5% humidity, a temperature range of 40°C–60°C, and (4500 ± 500) LX.
The last system is a liposomal one, and there are several approaches used to prepare berberine hydrochloride liposomes, including the thin film evaporation method, the active loading method, and a combination of the thin film evaporation and active loading methods. The thin film evaporation process could achieve a higher encapsulation efficiency, and the optimum manufacturing processes are characterized by 60°C of hatched temperature, 30 minutes of time, and 3.3 mg/mL of cholesterol concentration.52 An active loading method is better than passive loading with a higher entrapment efficiency.53 There are several factors to be considered, including addition sequence, incubation time, incubation temperature, pH value of the external water phase, and the particle size of the liposome. Changing the addition sequence can also achieve different entrapment efficiencies, as can increasing incubation time and temperature and decreasing the particle size.53 Chen et al used different individual variables and an orthogonal design to obtain the optimal preparation conditions for berberine hydrochloride liposomes, including incubation time and incubation temperature, proportional weights of drug and lipids, soybean phosphatidylcholine, and cholesterol.54 The results indicated that the average encapsulation efficiency of the optimized liposome was 78.51% ± 2.45%, with a size range of 2.2–3.5 μm. Based on the uniform design, berberine hydrochloride liposomes were prepared by thin film evaporation and an active loading method.55 An optimal formulation was established and a high encapsulation efficiency of 79.33% was obtained with a drug loading ratio of 30.21 and a size range of 2.2–3.8 μm. In vivo and in vitro studies were carried out to investigate the way in which liposomal berberine hydrochloride works. Gou et al studied the effects of berberine hydrochloride liposomes on the combination of impaired glucose tolerance and hyperlipidemia, suggesting that glucose and lipid metabolism was interfered with and progression from hyperlipidemia to type 2 diabetes was also prevented.56
Studies of the molecular mechanisms by which berberine hydrochloride affects lipid and glucose metabolism warrant further attention. Additionally, with the increasing research on reprogramming of cancer cell metabolism, including inhibition of glycolysis, impairment of mitochondrial function, and suppression of cell anabolism, it could be possible to reverse abnormal cancer cell metabolism in various ways, including blocking formation of the cell membrane and synthesis of macromolecules, and inhibiting growth and proliferation of cancer cells. During the process of interfering with cancer cell reprogramming, because of the alteration in the physiological properties of the cell, multidrug resistance, sensitivity of chemotherapeutic drugs, and patient quality of life could be ameliorated, which indicates the potential of berberine hydrochloride as an adjuvant drug in antineoplastic treatment. With effective troubleshooting of issues such as the hydrophobic properties, poor stability, and bioavailability of berberine hydrochloride, research on nanoparticulate delivery systems for this compound is being vigorously pursued. Furthermore, investigations in this field are likely to provide new insights into anticancer drug preparation in Chinese medicine.
This study was supported by the Macao Science and Technology Development Fund (029/2007/A2) and University of Macau Research Fund (UL016A/09-Y2/CMS/WYT01/ICMS).
Disclosure
The authors report no conflicts of interest in this work.
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